Protocol for detecting cytosol-retained proopiomelanocortin by fractionation and co-immunoprecipitation assay
Sang-Hyeon Mun, Cheol-Sang Hwang · 2026
Read Protocol for detecting cytosol-retained proopiomelanocortin by fractionation and co-immunoprecipitation assay by Sang-Hyeon Mun, Cheol-Sang Hwang (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13316517.txt.
Description
Sang-Hyeon Mun and Cheol-Sang Hwang share a 2026 protocol for finding a hormone parent protein, POMC, stuck in the wrong cell compartment. When its entry tag stays uncut, the protein piles up in the cell fluid instead of moving through the secretory path, a fault tied to stress and cell death.
The method first builds and checks an antibody that spots only the uncut tag form. Gentle digitonin treatment then frees the cell fluid while keeping organelles shut, and a stronger detergent opens the rest for comparison. Blotting and pull-down steps reveal the stuck protein, shown in human kidney-derived cells and adaptable to mouse brain cells. The recipe helps labs study protein traffic faults in disease.
Catalog record
| CREATOR | Sang-Hyeon Mun, Cheol-Sang Hwang |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 179736 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |