Selection and validation of novel DNA aptamer panel co-specific to Plasmodium falciparum and Plasmodium ovale lactate dehydrogenase
Liga Kunrade, Karlis Pleiko, Laura Sproge, Karina Goluba, Teodors Pantelejevs, Nathan Thompson Wright, Inara Akopjana, Janis Bogans, Kaspars Tars, Una Riekstina · 2026
Read Selection and validation of novel DNA aptamer panel co-specific to Plasmodium falciparum and Plasmodium ovale lactate dehydrogenase by Liga Kunrade, Karlis Pleiko, Laura Sproge, Karina Goluba, Teodors Pantelejevs, Nathan Thompson Wright, Inara Akopjana, Janis Bogans, Kaspars Tars, Una Riekstina (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13315843.txt.
Description
Liga Kunrade and colleagues select, in a 2026 study, a panel of DNA aptamers for malaria diagnosis that recognizes two parasite species. Point-of-care aptamer sensors could improve malaria detection, management, and eradication. Using staged evolution of ligands by cycled screening, the team selected short DNA aptamers against malaria lactate dehydrogenase, then validated the five abundant with a linked assay.
Binding affinities to the falciparum enzyme varied between 0.35 and 3.08 nanomolar. Trimming enhanced binding in most cases, with all shortened aptamers matching or beating full-length versions except Pf-LDH5t, which lost binding. Testing showed Pf-LDH1 and its shortened form recognized only falciparum enzyme, while Pf-LDH2, Pf-LDH3, Pf-LDH4, their shortened forms, and Pf-LDH5 recognized both falciparum and ovale enzymes. Crystal work on Pf-LDH4t revealed a binding site overlapping an aptamer's but with new interactions. The authors offer the panel for new malaria diagnostics.
Catalog record
| CREATOR | Liga Kunrade, Karlis Pleiko, Laura Sproge, Karina Goluba, Teodors Pantelejevs, Nathan Thompson Wright, Inara Akopjana, Janis Bogans, Kaspars Tars, Una Riekstina |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 48 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |