Integrated IP-MS and Optoproteomics Reveal Dynamic Remodeling of Protein Interaction Networks During NLRP3 Inflammasome Activation
Yuehui Zhang, Kairong Zhang, Chaozhi Jin, Limin Shang, Shanshan Wu, Mingyang Zhang, Yanzhi Yuan, Yuchen Liu, Jian Wang · 2026
Read Integrated IP-MS and Optoproteomics Reveal Dynamic Remodeling of Protein Interaction Networks During NLRP3 Inflammasome Activation by Yuehui Zhang, Kairong Zhang, Chaozhi Jin, Limin Shang, Shanshan Wu, Mingyang Zhang, Yanzhi Yuan, Yuchen Liu, Jian Wang (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13311160.txt.
Description
Yuehui Zhang and colleagues map in 2026 how the protein neighbourhood of the NLRP3 inflammasome reshuffles as this inflammation machine switches on inside immune cells. The inflammasome sensor and its partner protein ASC were fished from mouse macrophages at priming and activation stages, with partners identified by mass spectrometry. A light-based labelling method added spatial detail around ASC clusters.
Profiling found nearly fifteen hundred stimulus-linked interactions collapsing to about a thousand distinct partners, reorganised stage by stage. Both capture routes converged on IMMT, a protein of the inner mitochondrial membrane, as an activation-stage partner of sensor and adaptor alike. Removing IMMT made the inflammasome fire harder: more caspase cleavage, more inflammatory cytokine release, more cell rupture. IMMT thus acts as a brake on assembly. A stimulus-resolved interaction map gives drug hunters precise points where inflammation might be tuned down.
Catalog record
| CREATOR | Yuehui Zhang, Kairong Zhang, Chaozhi Jin, Limin Shang, Shanshan Wu, Mingyang Zhang, Yanzhi Yuan, Yuchen Liu, Jian Wang |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 54 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |