Multiparametric Flow Cytometry Panel for Characterization of Mouse T Cell Differentiation and NK Cell Maturation Following Inflammatory Challenge
Tim Bozic, Bostjan Markelc, Simona Kranjc Brezar, Ziva Pisljar, Tanja Jesenko, Maja Cemazar · 2026
Read Multiparametric Flow Cytometry Panel for Characterization of Mouse T Cell Differentiation and NK Cell Maturation Following Inflammatory Challenge by Tim Bozic, Bostjan Markelc, Simona Kranjc Brezar, Ziva Pisljar, Tanja Jesenko, Maja Cemazar (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13305854.txt.
Description
Tim Bozic and colleagues set out a 2026 laboratory protocol for profiling mouse lymph node cells with 14-colour flow cytometry. Lymph nodes guide immune responses, so detailed maps of their resident cells help explain how immunity is organised.
Steps cover finding nodes by anatomy, breaking tissue apart by hand and with enzymes, then staining and gating to separate helper and killer T cells by maturity stage. Killer-cell relatives called natural killer cells are also sorted by development stage. Although tuned for response after a bacterial challenge agent, the panel offers a reusable route for wider immune studies.
Catalog record
| CREATOR | Tim Bozic, Bostjan Markelc, Simona Kranjc Brezar, Ziva Pisljar, Tanja Jesenko, Maja Cemazar |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 62400 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |