Optimized Digestion Conditions for Membrane Protein Footprinting and Mass Spectrometry Analysis
Ming Cheng, Xinzhu Li, Lin Bai, Weikai Li, Michael L. Gross · 2026
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Description
Ming Cheng and colleagues tune protein digestion for studying membrane proteins in a 2026 methods study. These water-shy proteins form most drug targets, yet they resist the cutting steps that mass spectrometry analysis depends on.
A single two-sided membrane protein served as the test case while enzyme and additive pairs were compared. A filter-based cleanup removed detergents well before measurement. The best recipe covered over nine tenths of the protein sequence with pieces of useful length. Fancy breakable soaps proved needless for single-protein work. Careful cleanup, enzyme choice, and piece size matter most, the authors conclude.
Catalog record
| CREATOR | Ming Cheng, Xinzhu Li, Lin Bai, Weikai Li, Michael L. Gross |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 42422 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |