Thermodynamics of Binding Between Adeno-Associated Viruses and Heparin in Bulk and at Interfaces via Isothermal Titration Calorimetry
Elizabeth Adeogun, Jude C. Obijiaku, Ronny Horax, Kayla E. Daugherty, Joshua Sakon, Xianghong Qian, Barbara Knutson, Stephen E. Rankin, Karthik Nayani · 2026
Read Thermodynamics of Binding Between Adeno-Associated Viruses and Heparin in Bulk and at Interfaces via Isothermal Titration Calorimetry by Elizabeth Adeogun, Jude C. Obijiaku, Ronny Horax, Kayla E. Daugherty, Joshua Sakon, Xianghong Qian, Barbara Knutson, Stephen E. Rankin, Karthik Nayani (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13295711.txt.
Description
Elizabeth Adeogun and co-authors measure how gene therapy viruses grip heparin, in a 2026 study of binding heat. Adeno-associated viruses ferry genes into cells for therapy, and makers need better ways to purify them at scale. Heparin, a sugar chain, is a candidate grip for sorting, yet virus amounts are so low that standard heat tools struggle to read the binding.
The team set design rules that let a heat-based method, isothermal titration calorimetry, work at these low amounts. Grip strength rose as the heparin chains grew longer, and the team counted how many chains bind each virus. Buffer mix and acidity shifted the grip as well. The rules should help design sorting steps that catch the virus by its surface.
Catalog record
| CREATOR | Elizabeth Adeogun, Jude C. Obijiaku, Ronny Horax, Kayla E. Daugherty, Joshua Sakon, Xianghong Qian, Barbara Knutson, Stephen E. Rankin, Karthik Nayani |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 32 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |