Enriching Bacteria-Specific RNA From Host Samples Before NGS With Transcript-Capture
Eleanor I Lamont, Richard M Jones, Jessica Assadi, Shuyi Ma, David R Sherman · 2026
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Description
Eleanor I Lamont and coauthors give a 2026 protocol for pulling bacterial RNA out of host-heavy samples before sequencing. Pathogen signals drown in host RNA, and bought probes often miss parts of the transcriptome. Their transcript-capture method builds 150-base biotinylated probes at home from bacterial DNA spanning the whole genome, then fishes matching cDNA from standard libraries.
Enrichment tops 200-fold in lab, animal, and human infection samples. Gene coverage passes 80%, giving near-complete bacterial transcriptomes where noise once blocked analysis. A snapshot of what bacteria actually do inside a host can reveal weak points that pure lab cultures hide.
Catalog record
| CREATOR | Eleanor I Lamont, Richard M Jones, Jessica Assadi, Shuyi Ma, David R Sherman |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 43169 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |