High-Resolution Mapping of RNA–RNA Interactions Across the HIV-1 Genome With HicapR
Haobo Wang, Yan Zhang, Jingwan Han, Dejian Xie, Wenlong Shen, Ping Li, Jian You Lau, Jingyun Li, Lin Li, Grzegorz Kudla, Zhihu Zhao · 2026
Read High-Resolution Mapping of RNA–RNA Interactions Across the HIV-1 Genome With HicapR by Haobo Wang, Yan Zhang, Jingwan Han, Dejian Xie, Wenlong Shen, Ping Li, Jian You Lau, Jingyun Li, Lin Li, Grzegorz Kudla, Zhihu Zhao (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13293981.txt.
Description
Haobo Wang and colleagues present HiCapR in 2026, a protocol for mapping how viral RNA strands touch each other across a whole genome. RNA viruses fold into shapes that control infection and immune evasion. Older ligation methods map contacts broadly but miss rare targets in mixed samples, so capture steps were added to enrich viral pieces.
Workflow covers psoralen crosslinking in live virus and infected cells, cutting, joining close pieces, capture, sequencing, and computer analysis of joined reads. Applied to HIV-1, the method resolved the full genome layout, including pairing regions and long-range contacts. Prior validation on SARS-CoV-2 supports use for antiviral design.
Catalog record
| CREATOR | Haobo Wang, Yan Zhang, Jingwan Han, Dejian Xie, Wenlong Shen, Ping Li, Jian You Lau, Jingyun Li, Lin Li, Grzegorz Kudla, Zhihu Zhao |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 29366 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |