Genetic disruption of Capns1 impairs metastatic phenotypes in murine mammary carcinoma cells
Danielle Harper, Ivan Shapovalov, Samantha Cockburn, Anya Aaron, Yan Gao, Peter A Greer · 2026
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Description
Danielle Harper and colleagues disable a shared calpain subunit to test metastasis in mouse breast cancer cells, in 2026. Calpains are calcium-switched enzymes long suspected of helping tumors spread. Two main forms share one regulatory subunit, Capns1, so deleting its gene silences both. The team used CRISPR editing in a mouse mammary carcinoma line. They then measured invasion in dishes and lung spread in tumor-bearing mice.
Knockout cells invaded about fifty-three percent less than normal cells in three-dimensional tests. In the mouse model, tumors were removed and lungs checked ten days later for spread. Metastatic burden in the lungs fell by about sixty-eight percent with the knockout. Tumor growth was tracked separately from spread. The authors say the genetic result validates calpains one and two as anti-metastatic targets. No approved calpain blocker exists, which makes the genetic proof valuable.
Catalog record
| CREATOR | Danielle Harper, Ivan Shapovalov, Samantha Cockburn, Anya Aaron, Yan Gao, Peter A Greer |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 14 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |