A Unified Mass Spectrometry Workflow Integrating Bottom-Up and Native Top-Down Proteomics via Nanodroplet Enzymatic Digestion
Hsi-Wen Wang, Yong-Lun Hong, Ting-Yi Chiang, Szu-Hsueh Lai · 2026
Read A Unified Mass Spectrometry Workflow Integrating Bottom-Up and Native Top-Down Proteomics via Nanodroplet Enzymatic Digestion by Hsi-Wen Wang, Yong-Lun Hong, Ting-Yi Chiang, Szu-Hsueh Lai (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13276839.txt.
Description
Hsi-Wen Wang and colleagues unite two protein analysis methods in a single workflow, in a 2026 paper. Bottom-up proteomics chops proteins into peptides for identification, while native top-down work keeps whole proteins and assemblies intact. Each method normally needs its own preparation, so the two views rarely come from one run.
Digestion happens inside tiny spray droplets under native conditions, which speeds the enzyme's work dramatically. By tuning how far digestion proceeds, one experiment yields peptides, intact proteins, and assemblies together. Tests on myoglobin, casein variants, and the huge GroEL complex showed fast sequence coverage with structure preserved. The authors offer the platform for fuller protein characterisation in one pass.
Catalog record
| CREATOR | Hsi-Wen Wang, Yong-Lun Hong, Ting-Yi Chiang, Szu-Hsueh Lai |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 34 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |