A guide for establishing patient-derived organoids from bile samples obtained during endoscopic procedures and performing gene expression knockdown
Carla Rojo, Juan J. Vila, Laura Guembe, Amaia Arrubla-Gamboa, Vanesa Jusué-Irurita, Juan Carrascosa-Gil, María Rullan, Javier Rández-Garbayo, Maite G. Fernández-Barrena, Meritxell Huch, Jesús Urman, Matías A. Ávila, Carmen Berasain, Maria Arechederra · 2026
Read A guide for establishing patient-derived organoids from bile samples obtained during endoscopic procedures and performing gene expression knockdown by Carla Rojo, Juan J. Vila, Laura Guembe, Amaia Arrubla-Gamboa, Vanesa Jusué-Irurita, Juan Carrascosa-Gil, María Rullan, Javier Rández-Garbayo, Maite G. Fernández-Barrena, Meritxell Huch, Jesús Urman, Matías A. Ávila, Carmen Berasain, Maria Arechederra (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13260716.txt.
Description
Carla Rojo and colleagues published in 2026 a step-by-step guide for growing patient organoids from bile collected during endoscopy. Tissue for bile duct disease and its cancers is scarce, which limits laboratory models. Bile drawn during a routine scope procedure offers an easier source of living duct cells.
The protocol was tuned on samples from 21 patients with benign blockages or malignant strictures. Growable organoids arose in seventeen cases, with success across cancer types running high. Cultures from cancers mixed normal-looking and tumor-like growths at first, so the guide covers handpicking when a pure tumor line is wanted. Companion workflows deliver gene-silencing molecules into the cultures without flattening their three-dimensional shape. DNA profiling of paired bile and organoid samples proved workable, supporting wider laboratory adoption.
Catalog record
| CREATOR | Carla Rojo, Juan J. Vila, Laura Guembe, Amaia Arrubla-Gamboa, Vanesa Jusué-Irurita, Juan Carrascosa-Gil, María Rullan, Javier Rández-Garbayo, Maite G. Fernández-Barrena, Meritxell Huch, Jesús Urman, Matías A. Ávila, Carmen Berasain, Maria Arechederra |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 103 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |