Engineering of fructose-6-phosphate aldolase for one-carbon conversion to mannitol in a designed biotransformation system
Dandan Wang, Qianzhen Dong, Peng Chen, Yan Zeng, Yinlu Liu, Yuanxia Sun, Jianxin Tan, Jiangang Yang · 2026
Read Engineering of fructose-6-phosphate aldolase for one-carbon conversion to mannitol in a designed biotransformation system by Dandan Wang, Qianzhen Dong, Peng Chen, Yan Zeng, Yinlu Liu, Yuanxia Sun, Jianxin Tan, Jiangang Yang (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13259622.txt.
Description
Dandan Wang and colleagues rebuilt an enzyme that stitches carbon chains, guided by quantum-level detective work. Fructose-6-phosphate aldolase already helps turn methanol into sugars in test-tube cascades. Its inner mechanism was murky, blocking smart redesign until this 2026 study.
Calculations pinpointed one step, forming a Schiff-base link, as the bottleneck of the whole reaction. Mutations aimed at that step delivered variants working thirty-four times more efficiently than the natural enzyme. Dropped into a designed cascade, the improved enzyme converted methanol into mannitol at high yield. The work sharpens tools for carbon-neutral chemical manufacturing from one-carbon feedstocks.
Catalog record
| CREATOR | Dandan Wang, Qianzhen Dong, Peng Chen, Yan Zeng, Yinlu Liu, Yuanxia Sun, Jianxin Tan, Jiangang Yang |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 62 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |