Methodological Insights from Low-Vacuum SEM for Morphological Analysis of Schwann Cells on Electrospun Scaffolds
Paulina Salazar-Aguilar, Andrea Barrenechea Sánchez, Karina Godoy Sánchez, Paulina Martínez-Rodríguez, Dimitrius Leonardo Pitol, María Eugenia González-Quijón, Fernando José Dias · 2026
Read Methodological Insights from Low-Vacuum SEM for Morphological Analysis of Schwann Cells on Electrospun Scaffolds by Paulina Salazar-Aguilar, Andrea Barrenechea Sánchez, Karina Godoy Sánchez, Paulina Martínez-Rodríguez, Dimitrius Leonardo Pitol, María Eugenia González-Quijón, Fernando José Dias (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13259224.txt.
Description
Paulina Salazar-Aguilar and colleagues refine how to photograph nerve-support cells growing on plastic scaffolds. Schwann cells drive peripheral nerve repair, and judging their shape on electrospun mats matters for tissue engineering. Their 2026 pilot compares six preparation recipes under a low-vacuum electron microscope, no metal coating required.
Seeding more cells made far more of them visible on the scaffold surface. Marking the seeded side with graphite helped counts and preserved natural star-like shapes. Marker ink, by contrast, seemed to repel the cells. The team also codified a careful way to trace cell extensions in image software, aiding repeatable measurements.
Catalog record
| CREATOR | Paulina Salazar-Aguilar, Andrea Barrenechea Sánchez, Karina Godoy Sánchez, Paulina Martínez-Rodríguez, Dimitrius Leonardo Pitol, María Eugenia González-Quijón, Fernando José Dias |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 29 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |