Nanobody-based analysis of RhoGTPase stress response in Schizosaccharomyces pombe: Role of mtl2
Noureen Rasheed, XiZe Ali, Tariq Ali, Faiz Muhammad, Muhammad Ali Rasheed · 2026
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Description
Noureen Rasheed and colleagues screen nanobodies for control of a stress pathway in fission yeast in a 2026 study. Yeast cell walls face constant mechanical and chemical attack, and wall sensors relay damage reports to repair pathways. The sensor protein Mtl2 is one such reporter; yeast missing it die under the antifungal drug caspofungin, making them a clean test bed.
The team built an in-vivo screening platform in the sensor-deficient strain under lethal drug conditions, hunting nanobodies, small antibody fragments, that could rescue survival. One hit, clone C, improved survival of the mutant and modulated Rgf1, an activator in the wall-integrity pathway. Computer docking suggested clone C grips Rgf1 away from the sites where it engages its partner GTPase, implying a tuning rather than blocking action. The experiments position nanobody screening inside living cells as a way to find modulators of stress signaling.
Catalog record
| CREATOR | Noureen Rasheed, XiZe Ali, Tariq Ali, Faiz Muhammad, Muhammad Ali Rasheed |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 67 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |