Development of Fit-for-Purpose, High Quality Proficiency Samples for Interlaboratory Evaluation of RT-PCR Detection of HPAI H5N1 in Milk
Emily L Smith, Yuhan Jin, Megan R Miller, Kirstin Frost, Jodie Ulaszek, Sarah M Nemser, Laura B Goodman, Steffen Uhlig, Anja Schlierf, Karina Hettwer, Matthew Kmet, Andriy Tkachenko, Olgica Ceric, Gregory H Tyson, Ravinder Reddy · 2026
Read Development of Fit-for-Purpose, High Quality Proficiency Samples for Interlaboratory Evaluation of RT-PCR Detection of HPAI H5N1 in Milk by Emily L Smith, Yuhan Jin, Megan R Miller, Kirstin Frost, Jodie Ulaszek, Sarah M Nemser, Laura B Goodman, Steffen Uhlig, Anja Schlierf, Karina Hettwer, Matthew Kmet, Andriy Tkachenko, Olgica Ceric, Gregory H Tyson, Ravinder Reddy (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13246525.txt.
Description
Smith, Jin, Miller, and co-authors built reference samples for testing milk for bird flu, in this 2026 Food and Environmental Virology paper. With highly pathogenic avian influenza H5N1 turning up in dairy cattle, laboratories need to detect the virus in milk, and proficiency exercises check whether labs get the right answers. That requires spiked milk samples whose virus levels are known precisely and stay uniform.
The team used digital PCR to count viral genomes in their stock and samples exactly, then confirmed the standard USDA detection assay read them consistently. Replicate samples barely varied, no milk-matrix interference appeared, and levels stayed stable over time. Such well-characterized samples, they argue, make nationwide lab evaluations trustworthy.
Catalog record
| CREATOR | Emily L Smith, Yuhan Jin, Megan R Miller, Kirstin Frost, Jodie Ulaszek, Sarah M Nemser, Laura B Goodman, Steffen Uhlig, Anja Schlierf, Karina Hettwer, Matthew Kmet, Andriy Tkachenko, Olgica Ceric, Gregory H Tyson, Ravinder Reddy |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 37 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |