Biochemical characterisation of a new thermotolerant L-asparaginase produced by Cunninghamella echinulata PA3S12MM isolated from the Atlantic Forest
Simone Von Groll, Leticia Claudia da Silva, Pedro Henrique Nascimento Souza, Wállison Justino da Silva, Thaís Duarte Bifano, José Luis da Conceição Silva, Rita de Cássia Garcia Simão, Simão, Marina Kimiko Kadowaki, Alexandre Maller · 2026
Read Biochemical characterisation of a new thermotolerant L-asparaginase produced by Cunninghamella echinulata PA3S12MM isolated from the Atlantic Forest by Simone Von Groll, Leticia Claudia da Silva, Pedro Henrique Nascimento Souza, Wállison Justino da Silva, Thaís Duarte Bifano, José Luis da Conceição Silva, Rita de Cássia Garcia Simão, Simão, Marina Kimiko Kadowaki, Alexandre Maller (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13242390.txt.
Description
Von Groll's team in Paraná coaxed a heat-loving enzyme from an Atlantic Forest fungus. Screening plate assays and deep fermentation singled out Cunninghamella echinulata PA3S12MM. Its L-asparaginase splits asparagine into aspartate and ammonia. Glucose plus chicken-feather waste topped enzyme output over five days.
The partly cleaned enzyme peaked at neutral pH and 85 degrees Celsius. It favored asparagine by far, with a third-rate attack on glutamine. One agent boosted activity; manganese ions and two others suppressed it. Pharma and food industry uses, from leukemia therapy to acrylamide control, beckon. The fungus came from the forest.
Catalog record
| CREATOR | Simone Von Groll, Leticia Claudia da Silva, Pedro Henrique Nascimento Souza, Wállison Justino da Silva, Thaís Duarte Bifano, José Luis da Conceição Silva, Rita de Cássia Garcia Simão, Simão, Marina Kimiko Kadowaki, Alexandre Maller |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 33 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |