Uncovering functional variants using a high-efficiency PE3-based screening platform
Shisheng Huang, Wanyu Tao, Xiangyang Li, Junfan Guo, Yu Zhang, Guanglei Li, Pengfei Zhang, Guiquan Zhang, Gaoyang Li, Gaofeng Fan, Shang-Min Zhang, Xingxu Huang · 2026
Read Uncovering functional variants using a high-efficiency PE3-based screening platform by Shisheng Huang, Wanyu Tao, Xiangyang Li, Junfan Guo, Yu Zhang, Guanglei Li, Pengfei Zhang, Guiquan Zhang, Gaoyang Li, Gaofeng Fan, Shang-Min Zhang, Xingxu Huang (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13240188.txt.
Description
Huang and colleagues build a screening platform that makes prime editing, a precise DNA rewriting tool, fast enough to test many gene variants. Prime editing can fix single letters of DNA, but it edits too weakly for large screens that ask what thousands of variants do. Their platform, EvoPRIME, sharpens the process and enriches for cells that took the edit.
A processing protein trims the guide RNAs, and a glow-based sort keeps the successfully edited cells. The team reports efficiency matching the best current systems, without needing to disable the cell's repair guards. Sharp screens like this can finally sort harmful variants from harmless ones at scale.
Catalog record
| CREATOR | Shisheng Huang, Wanyu Tao, Xiangyang Li, Junfan Guo, Yu Zhang, Guanglei Li, Pengfei Zhang, Guiquan Zhang, Gaoyang Li, Gaofeng Fan, Shang-Min Zhang, Xingxu Huang |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 65 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |