Sequential in vivo CRISPR screens identify the clonal dominance of Nf1 loss in long-term hematopoiesis
Shiru Yuan, Qian Wu, Tong Yin, Lianting Chen, Baixuan Zhang, Pujiao Li, Xiaowei Xie, Guohuan Sun, Tao Cheng, Jun Wei, Hui Cheng · 2026
Read Sequential in vivo CRISPR screens identify the clonal dominance of Nf1 loss in long-term hematopoiesis by Shiru Yuan, Qian Wu, Tong Yin, Lianting Chen, Baixuan Zhang, Pujiao Li, Xiaowei Xie, Guohuan Sun, Tao Cheng, Jun Wei, Hui Cheng (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13221118.txt.
Description
Shiru Yuan and colleagues built a two-stage CRISPR screening system in living mice, reported in 2026, to find genes governing long-term blood stem cells. Single screens can miss how clones evolve over months. Their platform recovered guide RNAs after five months, then used pooled secondary transplants to watch clonal competition unfold.
One hit dominated: loss of Nf1, a known tumor suppressor gene. The first screen reproduced a state of excess blood cell production. After secondary transplant, that state advanced toward malignant blood disease. The design lets researchers follow the step from healthy stem cell function to cancer in one system, useful for studying leukemia origins.
Catalog record
| CREATOR | Shiru Yuan, Qian Wu, Tong Yin, Lianting Chen, Baixuan Zhang, Pujiao Li, Xiaowei Xie, Guohuan Sun, Tao Cheng, Jun Wei, Hui Cheng |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 44 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |