PCR-based detection and phylogenetic analysis of Candidatus Liberibacter asiaticus in citrus orchards across Nepal
Richa Giri, Bal Kumari Oliya, Siddartha Gautam, Krishna Das Manandhar, Nishant Kumar, Nishant Kumar, Nishant Kumar, Nishant Kumar · 2026
Read PCR-based detection and phylogenetic analysis of Candidatus Liberibacter asiaticus in citrus orchards across Nepal by Richa Giri, Bal Kumari Oliya, Siddartha Gautam, Krishna Das Manandhar, Nishant Kumar, Nishant Kumar, Nishant Kumar, Nishant Kumar (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13215486.txt.
Description
Richa Giri and co-authors report a 2026 survey of citrus greening disease across Nepal, using DNA testing to find the bacterium behind it. The disease, also called huanglongbing, is one of the most damaging threats to citrus crops worldwide.
The team tested 1,026 leaf samples from thirteen districts in six provinces with a PCR test aimed at the pathogen's 16S gene, and 255 samples came back positive. Sequencing of five samples showed the Nepali bacteria were over 99 percent similar to reference strains and closest to ones from India. The authors call for certified clean planting material and control of the psyllid insects that spread the disease.
Catalog record
| CREATOR | Richa Giri, Bal Kumari Oliya, Siddartha Gautam, Krishna Das Manandhar, Nishant Kumar, Nishant Kumar, Nishant Kumar, Nishant Kumar |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 78 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |