Protocol for isolation and characterization of small extracellular vesicles from human menstrual fluid using size-exclusion chromatography
Yiqun Tang, Ruihan Zhou, Caroline Frisendahl, Kristina Gemzell-Danielsson · 2026
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Description
Yiqun Tang and three co-authors publish a step-by-step protocol for harvesting tiny membrane bubbles, called small extracellular vesicles, from human menstrual fluid. The fluid is rich in these vesicles, shed by blood components and by cells of the womb lining and immune system. Such vesicles carry molecular cargo that could serve as biomarkers or treatments for uterine disorders, but only if isolation is clean and repeatable.
The method runs through dilution, gentle spinning, filtration, and a size-based column that separates vesicles from loose protein. The team then describes three checks on the pooled product: electron microscopy for shape, particle tracking for size and count, and protein blotting for marker identity. They report that the workflow yields vesicles of high quality suited to genetic, protein, and functional studies. The protocol is offered so other labs can reproduce the preparation exactly and compare results across studies.
Catalog record
| CREATOR | Yiqun Tang, Ruihan Zhou, Caroline Frisendahl, Kristina Gemzell-Danielsson |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 44 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |