A standardized single-tube 17-color spectral flow cytometry workflow for integrated immunophenotyping of human PBMCs and mixed co-culture systems
Klaudia Kiel, Marta Małuszek, Katarzyna Piwocka, Jakub Godlewski, Agnieszka Bronisz · 2026
Read A standardized single-tube 17-color spectral flow cytometry workflow for integrated immunophenotyping of human PBMCs and mixed co-culture systems by Klaudia Kiel, Marta Małuszek, Katarzyna Piwocka, Jakub Godlewski, Agnieszka Bronisz (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13194583.txt.
Description
Klaudia Kiel and co-authors publish a standardized recipe for reading seventeen immune markers on human blood cells in a single tube. Their 2026 methods paper serves spectral flow cytometry, which sorts cells by many fluorescent tags at once. More colors mean more chances for signals to bleed into each other, so the craft lies in control.
The protocol builds in similarity and spillover metrics, dye assignment by marker abundance, careful antibody titration, blank-anchored gates, and correction for the cells' own glow. Longitudinal controls keep runs comparable across months. A shared, quality-controlled recipe lets different labs' immunophenotyping actually agree, which multi-center immune studies require.
Catalog record
| CREATOR | Klaudia Kiel, Marta Małuszek, Katarzyna Piwocka, Jakub Godlewski, Agnieszka Bronisz |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 79 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |