Transposon insertion sequencing identifies novel genes involved in product synthesis in gas-fermenting Clostridium ljungdahlii
Zhiyi Zhang, Fengjuan Yang, Yihan Liu, Wei Ye, Dechen Jia, Yi Tian, Weihong Jiang, Qiyao Wang, Yang Gu · 2026
Read Transposon insertion sequencing identifies novel genes involved in product synthesis in gas-fermenting Clostridium ljungdahlii by Zhiyi Zhang, Fengjuan Yang, Yihan Liu, Wei Ye, Dechen Jia, Yi Tian, Weihong Jiang, Qiyao Wang, Yang Gu (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13188128.txt.
Description
Zhiyi Zhang and eight co-authors flood a gas-eating bacterium with random gene breaks to find which genes run its trade. The bacterium turns waste gases into ethanol and acetate, goods worth making. Past gene hunts in it were slow. Their 2026 study builds a genome-wide break library and reads it in bulk.
A jumping gene system seeded breaks across the genome, with a switch to clear the carrier later. Bulk reads then ranked genes by how much the breaks hurt growth on gas. Hand tests of top genes showed shifts in both goods the cell makes. The map is a shared tool for tuning the strain. The same path can hunt genes for other traits, the team says.
Catalog record
| CREATOR | Zhiyi Zhang, Fengjuan Yang, Yihan Liu, Wei Ye, Dechen Jia, Yi Tian, Weihong Jiang, Qiyao Wang, Yang Gu |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 61 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |