Preparation of cleared whole-mount urethra and urinary bladder from transcardially perfused mice for immunolabeling and analysis by CLSM
Uwe Pfeil, Felix Dettler, Alexander Perniss, Praju Vikas Anekal, Lora Bankova, Paula Montero LIopis, Klaus Deckmann · 2026
Read Preparation of cleared whole-mount urethra and urinary bladder from transcardially perfused mice for immunolabeling and analysis by CLSM by Uwe Pfeil, Felix Dettler, Alexander Perniss, Praju Vikas Anekal, Lora Bankova, Paula Montero LIopis, Klaus Deckmann (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13186176.txt.
Description
Pfeil and six colleagues give a protocol for preparing mouse bladder and urethra for microscopy in twenty twenty-six. Seeing cells in their three dimensional tissue setting aids understanding. Whole mount preparation keeps the organ intact instead of slicing it. Their method starts with mice perfused through the heart.
Steps cover dissection, tissue clearing, and antibody labelling. Cleared tissue becomes transparent for confocal laser scanning microscopy. Three dimensional processing of the images is also detailed. The protocol suits the urethra and urinary bladder of mice. It lets researchers map cell networks across whole organs. The authors point readers to an earlier study for full usage details.
Catalog record
| CREATOR | Uwe Pfeil, Felix Dettler, Alexander Perniss, Praju Vikas Anekal, Lora Bankova, Paula Montero LIopis, Klaus Deckmann |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 130 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |