Thermal optimized PCR coupled to CRISPR-Cas12a for rapid detection of blaOXA-1 resistance gene
Ana Quiroz-Huanca, Maryhory Vargas-Reyes, Juan Diego López, Kiara Flores-Jimenez, Sofia Saldarriaga-Morán, Karla Cifuentes, Roberto Alcántara, Iddya Karunasagar, Iddya Karunasagar, Iddya Karunasagar, Iddya Karunasagar · 2026
Read Thermal optimized PCR coupled to CRISPR-Cas12a for rapid detection of blaOXA-1 resistance gene by Ana Quiroz-Huanca, Maryhory Vargas-Reyes, Juan Diego López, Kiara Flores-Jimenez, Sofia Saldarriaga-Morán, Karla Cifuentes, Roberto Alcántara, Iddya Karunasagar, Iddya Karunasagar, Iddya Karunasagar, Iddya Karunasagar (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13178884.txt.
Description
Ana Quiroz-Huanca and co-authors build a fast test for a drug-resistance gene in a 2026 study. Drugs of the beta-lactam class are front-line cures, but enzymes that break them keep spreading. One gene family now holds more than 400 forms and is common in gut germs, while old tests are slow or need costly gear.
The team paired a tuned heat-cycle copy step with a CRISPR-based glow test for the target gene in gut germ samples. Settings for ramp speed and hold time were cut to a second each. The result is a quick lab test that may bring gene checks closer to clinics.
Catalog record
| CREATOR | Ana Quiroz-Huanca, Maryhory Vargas-Reyes, Juan Diego López, Kiara Flores-Jimenez, Sofia Saldarriaga-Morán, Karla Cifuentes, Roberto Alcántara, Iddya Karunasagar, Iddya Karunasagar, Iddya Karunasagar, Iddya Karunasagar |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 68 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |