A Scalable and Cost‐Effective In‐Line Barcoding Strategy for Standardized 16S rRNA Gene Amplicon Sequencing: Performance Evaluation and Bias Assessment
Lisa Jourdain, Pierre Rossi, Aline Charpagne, Emmanuelle Chevalier, Viviane Praz, Julien Marquis, Johann Weber, Wenyu Gu · 2026
Read A Scalable and Cost‐Effective In‐Line Barcoding Strategy for Standardized 16S rRNA Gene Amplicon Sequencing: Performance Evaluation and Bias Assessment by Lisa Jourdain, Pierre Rossi, Aline Charpagne, Emmanuelle Chevalier, Viviane Praz, Julien Marquis, Johann Weber, Wenyu Gu (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13159520.txt.
Description
Lisa Jourdain and seven co-authors present a cheaper way to tag many samples at once for gene sequencing. Their method, from two thousand twenty-six, reads a gene found in all bacteria and archaea. Tagging each sample lets a lab run many samples in one run and still tell them apart.
The team built and tested more than a hundred tagged primer pairs for each group. The tags worked well, with few dropouts and no clear bias in which microbes they found. They picked final sets of ninety-six pairs for each group. They also share their data, code, and a way to check for bias. Labs can use the sets for large studies of microbe life.
Catalog record
| CREATOR | Lisa Jourdain, Pierre Rossi, Aline Charpagne, Emmanuelle Chevalier, Viviane Praz, Julien Marquis, Johann Weber, Wenyu Gu |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 60 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |