A rapid multiplex platform for simultaneous detection of chikungunya virus, dengue virus, and dengue serotyping based on isothermal amplification and lateral flow dipsticks
Gaowen Liu, Xinlin Wu, Yingchao Chang, Mengyuan Zheng, Li Liu, Xueshan Xia, Yue Feng · 2026
Read A rapid multiplex platform for simultaneous detection of chikungunya virus, dengue virus, and dengue serotyping based on isothermal amplification and lateral flow dipsticks by Gaowen Liu, Xinlin Wu, Yingchao Chang, Mengyuan Zheng, Li Liu, Xueshan Xia, Yue Feng (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13156856.txt.
Description
Gaowen Liu and co-authors build a fast test that spots two mosquito-borne viruses at once and then sorts one of them by strain. Dengue and chikungunya cause similar fevers, so telling them apart quickly shapes care and outbreak control. Their method copies viral genes at one steady warmth, using an enzyme method called RT-MIRA, and reads the result by glow or on a paper dipstick.
The paired test showed no false hits against 35 related germs and could find about ten to thirteen copies per microliter. On 236 patient samples it caught 96 percent of dengue and 89 percent of chikungunya cases, with no false alarms, and agreed closely with the standard lab test. A second step sorted dengue samples into strains. The authors say the kit suits front-line testing where labs are scarce.
Catalog record
| CREATOR | Gaowen Liu, Xinlin Wu, Yingchao Chang, Mengyuan Zheng, Li Liu, Xueshan Xia, Yue Feng |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 41 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |