An Advanced Single-Cell RNA Sequencing (scRNA-seq) Protocol Utilizing Custom-Designed Multiplexing
Feng Gao, Xujie Liu, Fan Sun, Yadong Xiao, Gutian Xiao, Zhaoxia Qu · 2026
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Description
Feng Gao and five co-authors present a lab protocol for reading gene activity in single cells from several samples at once, in a 2026 paper. Mixing samples in one run saves cost and cuts batch effects, but the standard tagging kits fail in some mouse strains.
The problem is a mismatch between the kit's antibodies and the surface marks of those strains. The authors instead tag cells with a cocktail of antibodies carrying biotin, linked to barcode strands through a connector protein. They proved the method on lung tissue from the strain that defeats the kits, producing clean single-cell libraries. The scheme also labels surface cells that standard tags miss, and it pairs with immune profiling in the same run.
Catalog record
| CREATOR | Feng Gao, Xujie Liu, Fan Sun, Yadong Xiao, Gutian Xiao, Zhaoxia Qu |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 32 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |