Evaluation of Bna.SCT and Bna.REF1 as Target Genes to Reduce Sinapine in Rapeseed Using a Protoplast‐Based CRISPR RNP Approach
Oliver Moss, Xueyuan Li, Selvaraju Kanagarajan, Eu Sheng Wang, Emelie Ivarson, Li‐Hua Zhu · 2026
Read Evaluation of Bna.SCT and Bna.REF1 as Target Genes to Reduce Sinapine in Rapeseed Using a Protoplast‐Based CRISPR RNP Approach by Oliver Moss, Xueyuan Li, Selvaraju Kanagarajan, Eu Sheng Wang, Emelie Ivarson, Li‐Hua Zhu (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13155187.txt.
Description
Oliver Moss and co-authors test two gene targets for lowering a bitter compound in rapeseed, in a 2026 study. Rapeseed meal is rich in protein, but sinapine cuts its value as animal feed by blocking nutrient uptake and hurting taste. Past breeding barely dented sinapine levels. The team used CRISPR editing delivered as ready-made protein complexes into single plant cells, a route that leaves no foreign DNA in the final plants.
Edits landed in twenty-two to sixty-three percent of cases, often in all four copies of a target gene at once. Seeds from one target line held up to thirty-eight percent less sinapine, and the other up to seventy-seven percent less. Plant growth looked normal. The results mark both genes as sound targets.
Catalog record
| CREATOR | Oliver Moss, Xueyuan Li, Selvaraju Kanagarajan, Eu Sheng Wang, Emelie Ivarson, Li‐Hua Zhu |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 32 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |