Customizable FDM-based zebrafish larva mold for live imaging

Marcela Xiomara Rivera Pineda, Jaakko Lehtimäki, Guillaume Jacquemet · 2026

Read Customizable FDM-based zebrafish larva mold for live imaging by Marcela Xiomara Rivera Pineda, Jaakko Lehtimäki, Guillaume Jacquemet (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13148467.txt.

Description

Marcela Xiomara Rivera Pineda, Jaakko Lehtimaki, and Guillaume Jacquemet present a customisable three-dimensional printed mould for orienting zebrafish larvae during live imaging. Their 2026 methods article improves on standard agarose mounting, which makes precise orientation difficult.

The mould is printed by fused deposition modelling and coated with resin for a smoother surface. It creates consistent larva-shaped wells that hold larvae in a belly-to-back orientation. Designed for standard glass-bottom dishes, the moulds are cheap to print on desktop machines. Open design files let users adapt them to different imaging setups. The approach enables reliable long-term imaging of multiple larvae, extending low-cost manufacturing in zebrafish workflows.

Catalog record

CREATORMarcela Xiomara Rivera Pineda, Jaakko Lehtimäki, Guillaume Jacquemet
YEAR2026
COLLECTIONPAPERS
FORMATTXT
FILE SIZE27 KB
VERSION1.0
LICENSECC BY 4.0
RIGHTSCC BY
SOURCEEurope PMC

Source: https://europepmc.org/articles/PMC13148467

Browse the full library · About the Tower of Babel