Molecular characterization of Cdh12-SCON conditional knockout mice reveals unexpected splicing changes
Mayke A. C. ten Hoor, Margot M. Linssen, Conny M. Brouwers, Jill W. C. Claassens, Jaap Mulder, Peter Hohenstein · 2026
Read Molecular characterization of Cdh12-SCON conditional knockout mice reveals unexpected splicing changes by Mayke A. C. ten Hoor, Margot M. Linssen, Conny M. Brouwers, Jill W. C. Claassens, Jaap Mulder, Peter Hohenstein (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13135578.txt.
Description
Mayke ten Hoor and five co-authors examine in 2026 at the molecular level a conditional knockout mouse built to study Cdh12, a gene tied to kidney malformation. The SCON system plants a short artificial intron meant to vanish during splicing until a trigger locks it in and kills the gene. Earlier checks judged the system mostly by visible traits.
Both the resting and triggered forms of the gene unexpectedly skipped a neighboring exon, shifting the reading frame and cutting message levels by about a quarter. The resting allele was therefore not silent as designed. Careful molecular audits like this one matter before phenotype results from fast knockout systems are trusted, since hidden splicing damage can mimic or mask a gene's true role.
Catalog record
| CREATOR | Mayke A. C. ten Hoor, Margot M. Linssen, Conny M. Brouwers, Jill W. C. Claassens, Jaap Mulder, Peter Hohenstein |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 41 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |