Development and application of ActA-based competitive ELISA for the specific diagnosis of ovine listeriosis
Chao Chen, Beiru Wang, Mengdi Zhao, Fanzeng Meng, Suojun Jiang, Xin’an Jiao, Yuelan Yin · 2025
Read Development and application of ActA-based competitive ELISA for the specific diagnosis of ovine listeriosis by Chao Chen, Beiru Wang, Mengdi Zhao, Fanzeng Meng, Suojun Jiang, Xin’an Jiao, Yuelan Yin (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12888685.txt.
Description
Chao Chen and co-authors developed in 2025 a blood test for listeriosis in sheep. The disease, caused by Listeria monocytogenes, threatens livestock and people alike. Their assay is a competitive ELISA built on the bacterial protein ActA, using a labeled monoclonal antibody. Every setting of the kit, from coating dose to cutoff, was tuned in the lab.
At the chosen cutoff the test caught every true positive and cleared over ninety-three percent of negatives. It ignored sera raised against other common pathogens. Agreement with a commercial kit reached about ninety-six percent, with steady repeat results. Screening 930 sheep sera then found a quarter positive for past infection. The authors offer the kit as a specific, sensitive tool for flock surveillance. Herd health programs are the obvious users.
Catalog record
| CREATOR | Chao Chen, Beiru Wang, Mengdi Zhao, Fanzeng Meng, Suojun Jiang, Xin’an Jiao, Yuelan Yin |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 31 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |