Enzyme-assisted nanopore sensor for sensitive and multiplexed detection of microRNAs
Xiaobin Huang, Yakun Yi, Ke Zhou, Lei Liu, Hai-Chen Wu · 2025
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Description
Tiny RNA snippets called microRNAs signal many diseases, but they float in blood at vanishingly low levels. Xiaobin Huang, Yakun Yi, Ke Zhou, Lei Liu, and Hai-Chen Wu build a sensor that can find several of them at once. Their device pairs magnetic beads coated with capture strands to an enzyme that amplifies the signal, read by a nanopore.
When a target microRNA binds, it opens the capture strand and lets an enzyme-tagged second strand attach. The enzyme then reshapes small peptide probes, and each reshaping leaves a distinct electrical fingerprint as it passes the pore. Magnetic cleanup keeps background noise low. The sensor reads three targets across a wide range, down to femtomolar levels. The authors see it as a strong candidate for early disease diagnosis.
Catalog record
| CREATOR | Xiaobin Huang, Yakun Yi, Ke Zhou, Lei Liu, Hai-Chen Wu |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 29 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |