Enhanced genetic stability and expression control in growth-decoupled continuous two-stage E. coli fermentations using plasmid-dependent thyA auxotrophic selection
Florian Simon, Patrick Stargardt, Natalia Danielewicz, Florian Weiß, Johanna Berein, Gerald Striedner, Juergen Mairhofer · 2025
Read Enhanced genetic stability and expression control in growth-decoupled continuous two-stage E. coli fermentations using plasmid-dependent thyA auxotrophic selection by Florian Simon, Patrick Stargardt, Natalia Danielewicz, Florian Weiß, Johanna Berein, Gerald Striedner, Juergen Mairhofer (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12853824.txt.
Description
Florian Simon and co-authors published this study in 2025. It steadies engineered gut bacteria for long continuous fermentation. Such runs promise efficient bioproduction, but the inserted DNA circles, called plasmids, get lost over time. Mutations and stray early expression also creep in. The team's fix makes cell survival depend on keeping the plasmid. They deleted a gene the cells need, then put that gene on the plasmid alone.
Extra design tweaks curbed plasmid loss and silenced output during seed phases. Small-scale screens confirmed even, stable producer populations. Two best designs then ran in continuous two-stage setups for over a thousand hours. Output, plasmid levels, and cell mass held steady throughout. The authors offer the system as an antibiotic-free platform for durable industrial production.
Catalog record
| CREATOR | Florian Simon, Patrick Stargardt, Natalia Danielewicz, Florian Weiß, Johanna Berein, Gerald Striedner, Juergen Mairhofer |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 86 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |