Evaluation of the influence of RNA extraction methods on the detection of citrus viruses and viroids
Madelein Dippenaar, Hans Jacob Maree, Rachelle Bester · 2025
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Description
Madelein Dippenaar, Hans Jacob Maree, and Rachelle Bester weigh how RNA extraction method sways the spotting of citrus viruses and viroids. Certification and disease control rest on exact pathogen tests, and extraction is their base step. The study came out in twenty twenty-five.
Plants of two citrus types bore many viruses and viroids. Bark and leaf samples went through four extraction protocols. Extracts were read by a gene copy test, with deep sequencing to confirm. A second round resampled leaves and added a fifth protocol and a tweaked one. Protocols were judged by pathogen counts and data checks, with housekeeping genes as anchors.
Catalog record
| CREATOR | Madelein Dippenaar, Hans Jacob Maree, Rachelle Bester |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 91 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |