Development of three-colour FRET cascade for force sensing of the putative RIAM-vinculin interaction in fibroblasts
Conor A Treacy, Tommy L Pallett, Tam T T Bui, Simon P Poland, Mark A Pfuhl, Maddy Parsons, Simon M Ameer‑Beg · 2025
Read Development of three-colour FRET cascade for force sensing of the putative RIAM-vinculin interaction in fibroblasts by Conor A Treacy, Tommy L Pallett, Tam T T Bui, Simon P Poland, Mark A Pfuhl, Maddy Parsons, Simon M Ameer‑Beg (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12828031.txt.
Description
Conor Treacy and co-authors built a three-color light-based sensor to test whether two proteins meet and pull on each other inside living cells. The proteins, RIAM and vinculin, sit in focal adhesions, the spots where a cell grips its surface. Their method passes energy between three glowing tags in a chain, read out by timing single photons, so meetings and forces can be mapped in space and time.
The team checked the sensor with purified proteins, light spectra, structural models, and electron microscopy. In mouse cells lacking vinculin, the sensor showed RIAM binding to one end of vinculin at the grip spots. Force-sensing versions read an average pull of about three piconewtons per adhesion, matching vinculin's known role in sensing force.
Catalog record
| CREATOR | Conor A Treacy, Tommy L Pallett, Tam T T Bui, Simon P Poland, Mark A Pfuhl, Maddy Parsons, Simon M Ameer‑Beg |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 50 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |