Structural basis of dimerization and cascade formation by Cas5
Yong Jun Kang, Hyun Ji Ha, Hyo Been Jin, So Yeon Lee, Hyun Ho Park · 2025
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Description
Yong Jun Kang and co-authors wrote a structural study from 2025 on Cas5, a building block of a bacterial immune complex. CRISPR systems defend bacteria against viruses, and their large Cascade assemblies scout for invader DNA. Many subtype parts still lack clear structures. The team solved the crystal structure of Cas5 from a cattle bacterium that carries two CRISPR types, and probed how the protein behaves in solution.
The protein pairs up into a dimer, held by key contacts including one charged bridge between two residues. Comparisons with relatives and with predicted models suggest the pairing is shared across species. One loop in the structure is unusually loose. The authors propose it reshapes when the full Cascade assembles, gripping partner proteins and the guide RNA. The picture gives the field a firmer grip on how this Cascade kind is built.
Catalog record
| CREATOR | Yong Jun Kang, Hyun Ji Ha, Hyo Been Jin, So Yeon Lee, Hyun Ho Park |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 42 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |