1,3-propanediol production by Klebsiella pneumoniae ∆dhaM∆ptsG∆glpK using glucose and glycerol as co-substrates
Shaoqi Sun, Weiyan Jiang, Yaoyu Cai, Wenqi Wang, Xinjie Bian, Taiyu Liu, Marina Tišma, Dexin Wang, Jian Hao · 2025
Read 1,3-propanediol production by Klebsiella pneumoniae ∆dhaM∆ptsG∆glpK using glucose and glycerol as co-substrates by Shaoqi Sun, Weiyan Jiang, Yaoyu Cai, Wenqi Wang, Xinjie Bian, Taiyu Liu, Marina Tišma, Dexin Wang, Jian Hao (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12821955.txt.
Description
Shaoqi Sun and co-authors engineer a gut bacterium to make a bulk chemical in work dated 2025. The chemical, 1,3-propanediol, is made from glycerol by Klebsiella pneumoniae. Part of the glycerol must burn to keep a cell fuel in balance, which caps the yield. The team blocks burn paths so all glycerol can go to product, with glucose burning to supply the fuel.
Glucose at first blocked glycerol use, so genes tied to that block were cut. One cut kept the product path strong while the burn path stayed shut. Fed in a half-to-one glucose mix, the strain made a high yield in vat tests. Mild air levels worked best.
Catalog record
| CREATOR | Shaoqi Sun, Weiyan Jiang, Yaoyu Cai, Wenqi Wang, Xinjie Bian, Taiyu Liu, Marina Tišma, Dexin Wang, Jian Hao |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 55 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |