An optimized protocol for plant extracellular vesicles isolation from Ophiopogon japonicus root: a comparative evaluation based on miRNA cargo
Yang Xiao, Liqi Feng, Xin Zhao, Siyu Chen, Fengqi Lv, Zihan Li, Qi Zheng, Tao Zhou, Yuntong Ma, Binjie Xu · 2025
Read An optimized protocol for plant extracellular vesicles isolation from Ophiopogon japonicus root: a comparative evaluation based on miRNA cargo by Yang Xiao, Liqi Feng, Xin Zhao, Siyu Chen, Fengqi Lv, Zihan Li, Qi Zheng, Tao Zhou, Yuntong Ma, Binjie Xu (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12821176.txt.
Description
Plants ship tiny bubble-like parcels between cells, and those parcels carry microRNAs that may even speak to animal cells. Yang Xiao and co-authors build a better way to harvest the parcels from a tough source: the tuberous roots of a medicinal lily relative, Ophiopogon japonicus. The roots are dense and full of starchy contaminants that spoil standard preparations.
Their recipe pairs enzyme pre-treatment, which loosens the root's cell walls, with spinning steps refined beyond the usual protocol. A dense-liquid layer step with an early clearing pass removed debris best. The winning method gave more parcels and cleaner ones than the standard routes. Sequencing the parcels' small RNAs then profiled their cargo. The authors offer the pipeline to other hard plant tissues.
Catalog record
| CREATOR | Yang Xiao, Liqi Feng, Xin Zhao, Siyu Chen, Fengqi Lv, Zihan Li, Qi Zheng, Tao Zhou, Yuntong Ma, Binjie Xu |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 56 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |