Improving biocide evaluation using propidium monoazide (PMA) viability staining technique
Xiang Shi, Hasrizal Abd Rahman, Julia R de Rezende · 2025
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Description
Xiang Shi, Hasrizal Abd Rahman, and Julia de Rezende improve how labs judge germ-killing chemicals in a 2025 study. DNA tests are the norm for counting microbes after a dose, but DNA lingers in dead cells, so live counts come out too high. A dye called PMA can block the dead cells' DNA.
The team dosed an oilfield microbe mix with two common biocides, then ran the standard DNA workflow with the dye added first. Live counts fell by at least tenfold against the no-dye read, a truer picture of the kill. The tweak suits energy sites that fight corrosion and fouling.
Catalog record
| CREATOR | Xiang Shi, Hasrizal Abd Rahman, Julia R de Rezende |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 49 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |