Chemically-inducible CRISPR/Cas9 circuits for ultra-high dynamic range gene perturbation
Rajini Srinivasan, Tao Sun, Azalia Sandles, Diana Wu, Liang Wang, Hetal Patel, Ryan Pabalate, Maram Bader, Amy Heidersbach, Clark Ho, Shiqi Xie, Andrew Ng, Benjamin Haley · 2025
Read Chemically-inducible CRISPR/Cas9 circuits for ultra-high dynamic range gene perturbation by Rajini Srinivasan, Tao Sun, Azalia Sandles, Diana Wu, Liang Wang, Hetal Patel, Ryan Pabalate, Maram Bader, Amy Heidersbach, Clark Ho, Shiqi Xie, Andrew Ng, Benjamin Haley (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12804943.txt.
Description
Rajini Srinivasan and co-authors build tighter switches for gene editing in cells in a 2025 paper. The editing tool can model disease and reveal gene roles, but a common switch leaks, causing edits when it should stay off. Leaks can harm the cell genome over time, and the on state can also be weak. The team combined two ways to hold the tool down when off.
Their all in one system showed a very wide gap between off and on states across cell lines and targets. A second switch based on a splicing drug gave low background and strong action. A third version silenced genes without cutting DNA. Readers learn how tighter control may make cell studies of key genes safer and cleaner.
Catalog record
| CREATOR | Rajini Srinivasan, Tao Sun, Azalia Sandles, Diana Wu, Liang Wang, Hetal Patel, Ryan Pabalate, Maram Bader, Amy Heidersbach, Clark Ho, Shiqi Xie, Andrew Ng, Benjamin Haley |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 89 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |