A simplified low-cost and reliable plant genomic DNA extraction method for PCR-based genotyping and screening
Cai-yun Yang, Duncan Scholefield, Stephen Ashling, Surbhi Grewal, Ian P King, Julie King · 2025
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Description
Cai-yun Yang and five colleagues present a cheap, one-tube method to pull DNA from plant tissue, in a twenty twenty-five paper. Standard methods are slow, pricey, and wasteful of plastic. Their method skips tube transfers, cutting handling errors and time. It yielded DNA pure enough for standard gene tests in wheat and its wild crosses.
Tests in maize, a model weed, and tomato confirmed wide use. Dried or frozen tissue gave more DNA than fresh. Two simple salt buffers both worked. The authors pitch the method for breeding programs that screen many plants, while noting it is not for jobs needing ultra-pure DNA.
Catalog record
| CREATOR | Cai-yun Yang, Duncan Scholefield, Stephen Ashling, Surbhi Grewal, Ian P King, Julie King |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 24 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |