Enhanced methanol tolerance and utilization in Pichia pastoris revealed by adaptive laboratory evolution
Junyeob Kim, Sang Woo Seo · 2025
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Description
Junyeob Kim and Sang Woo Seo present a 2025 study on breeding a yeast that thrives on methanol, a simple carbon feedstock with promise for green manufacturing. The yeast Pichia pastoris is a natural methanol user and a candidate factory strain. Methanol's toxicity, though, caps how much can be fed at once, choking production.
By passing the yeast through rising methanol levels over many generations, they evolved a strain that grows well in seven percent methanol, a level the parent strain cannot survive. Genome reading found the changes behind the gain. Moving two single gene changes into the parent yeast confirmed each one lifts growth under methanol stress.
Catalog record
| CREATOR | Junyeob Kim, Sang Woo Seo |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 44 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |