Artifact-free preparation of biological samples for SEM by optimized water freeze-drying
Hideki Ishida, Naoya Fukuda, Maho Shimada, Kousei Yamamoto, Liudmyla Gaponova, Rina Higuchi, Andrii Kolosiuk, Ryo Hoshina, Mikihiko Arikawa, Yasuhiro Fukuda, MD Shafiq Islam, Terue Harumoto, Yumeng Wan, Toshiki Itoh, Yoko Inai, Asuka Takeishi, Hitoshi Aonuma, Takuto Kikuchi, Shijo Nishigori, Tadashi · 2025
Read Artifact-free preparation of biological samples for SEM by optimized water freeze-drying by Hideki Ishida, Naoya Fukuda, Maho Shimada, Kousei Yamamoto, Liudmyla Gaponova, Rina Higuchi, Andrii Kolosiuk, Ryo Hoshina, Mikihiko Arikawa, Yasuhiro Fukuda, MD Shafiq Islam, Terue Harumoto, Yumeng Wan, Toshiki Itoh, Yoko Inai, Asuka Takeishi, Hitoshi Aonuma, Takuto Kikuchi, Shijo Nishigori, Tadashi (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12780166.txt.
Description
Hideki Ishida and co-authors dry living cells for electron microscopy in a 2025 methods paper. Drying soft cells for scanning electron microscopy usually needs chemical fixation. Water freeze drying was dismissed because ice crystals shred cell structure. Their optimized version skips fixation entirely.
Live cells in water are frozen against a copper block at minus eighty degrees. A guide cylinder keeps the contact flat and precise. Success rose from about ten percent to twenty or thirty percent. Transmission imaging confirmed far fewer ice artifacts inside cells. Preparation takes only two to three hours. Protists and other organisms imaged sharply with the method. The protocol is standardized for routine lab use. It opens cheap, fast microscopy for delicate specimens.
Catalog record
| CREATOR | Hideki Ishida, Naoya Fukuda, Maho Shimada, Kousei Yamamoto, Liudmyla Gaponova, Rina Higuchi, Andrii Kolosiuk, Ryo Hoshina, Mikihiko Arikawa, Yasuhiro Fukuda, MD Shafiq Islam, Terue Harumoto, Yumeng Wan, Toshiki Itoh, Yoko Inai, Asuka Takeishi, Hitoshi Aonuma, Takuto Kikuchi, Shijo Nishigori, Tadashi |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 43 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |