Controlling Intramolecular Rotation with Five-Membered Heterocycles Facilitates the Design of Highly Cell-Permeable Xanthene-Based Fluorogenic Probes
Shahi Imam Reja, Yuichiro Hori, Youhei Takeda, Miyako Nishiura, Masafumi Minoshima, Kazuya Kikuchi · 2025
Read Controlling Intramolecular Rotation with Five-Membered Heterocycles Facilitates the Design of Highly Cell-Permeable Xanthene-Based Fluorogenic Probes by Shahi Imam Reja, Yuichiro Hori, Youhei Takeda, Miyako Nishiura, Masafumi Minoshima, Kazuya Kikuchi (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12766722.txt.
Description
Shahi Imam Reja and co-authors design glow in the dark probes that enter cells easily and switch on without washing. Their 2025 study builds on xanthene dyes, a family used in imaging. Good probes need strong signal, low background, and easy cell entry. The team placed five membered rings, furan or thiophene, at a key spot on the dye core. That change controls how a bond inside the molecule rotates.
Rotation is blocked in thick fluids or when the probe binds a target, and that block turns the glow on. The trick does not rely on the ring opening that older dyes use. The design extended to two related dye families. Probes were built for three self labeling protein tags. One probe filmed a cell structure forming in real time. Variants aimed at two drug targets were also made by attaching known inhibitors.
Catalog record
| CREATOR | Shahi Imam Reja, Yuichiro Hori, Youhei Takeda, Miyako Nishiura, Masafumi Minoshima, Kazuya Kikuchi |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 54 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |