Accessing Self-Illuminated, Luminescent Lanthanide Probes by Enzymatic Radiophosphorylation
Georgia G. Sands, Yichong Lao, M. Andrey Joaqui-Joaqui, Xuhui Huang, Eszter Boros · 2025
Read Accessing Self-Illuminated, Luminescent Lanthanide Probes by Enzymatic Radiophosphorylation by Georgia G. Sands, Yichong Lao, M. Andrey Joaqui-Joaqui, Xuhui Huang, Eszter Boros (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12754790.txt.
Description
Georgia Sands and co-authors describe self-illuminated lanthanide probes made by enzymatic radiophosphorylation in a 2025 paper. The team built terbium and europium complexes attached to a kinase-substrate peptide. An enzyme then adds phosphorus-32, a potent Cerenkov emitter, to form a glowing metal-bound peptide.
The design was tuned to hold one inner-sphere donor, with a specific macrocycle giving a selective turn-on signal when phosphate displaces a water molecule. The lead peptide showed a selective glow increase and a high radiochemical yield. Optical imaging reached the best sensitivity yet reported for a lanthanide probe with standard tools. The work opens a path to sensitive imaging of kinase activity in living systems.
Catalog record
| CREATOR | Georgia G. Sands, Yichong Lao, M. Andrey Joaqui-Joaqui, Xuhui Huang, Eszter Boros |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 36 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |