Engineered AcrIIA5 for optogenetic control of CRISPR‐Cas9‐based genome editing
Qi Chen, Jia Yao, Yingfan Lu, Ruikang Qiu, Zixin Deng, Yuhui Sun · 2025
Read Engineered AcrIIA5 for optogenetic control of CRISPR‐Cas9‐based genome editing by Qi Chen, Jia Yao, Yingfan Lu, Ruikang Qiu, Zixin Deng, Yuhui Sun (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12754625.txt.
Description
Qi Chen and co-authors built a light switch that can pause the gene editor CRISPR-Cas9, in this 2025 study. Editor blockers exist, but each fits only one editor type. The team slotted a blue-light sensor into a blocker with a broad reach.
The new tool held four editor types in check, releasing them only under blue light. A second design added a light-triggered self-destruct tag to the blocker and also worked. Switchable control promises safer, better timed edits in cells. The authors offer both designs as templates for tuning other blockers. Lab tests in cells backed each claim.
Catalog record
| CREATOR | Qi Chen, Jia Yao, Yingfan Lu, Ruikang Qiu, Zixin Deng, Yuhui Sun |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 39 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |