Transformation of Tn7 insertion elements across strains of Vibrio fischeri
Andrew G. Cecere, Chris Muriel-Mundo, Derek J. Fisher, Tim I. Miyashiro, Claudia Isabella Pogoreutz, Claudia Isabella Pogoreutz, Claudia Isabella Pogoreutz · 2025
Read Transformation of Tn7 insertion elements across strains of Vibrio fischeri by Andrew G. Cecere, Chris Muriel-Mundo, Derek J. Fisher, Tim I. Miyashiro, Claudia Isabella Pogoreutz, Claudia Isabella Pogoreutz, Claudia Isabella Pogoreutz (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12752967.txt.
Description
Cecere and co-authors share a protocol for moving a standard genetic tool into wild strains of a glowing marine bacterium. The bacterium, Vibrio fischeri, lives in symbiosis with the Hawaiian bobtail squid. Tools built for the lab strain often fail in its wild relatives.
Their workaround first inserts the desired DNA into the lab strain at the usual landing site. DNA from that strain then transforms the target strain, whose natural uptake is switched on. As proof, they restored a broken gene in another strain using an inducible copy. The authors offer the method to anyone studying symbiosis genes in hard-to-edit strains.
Catalog record
| CREATOR | Andrew G. Cecere, Chris Muriel-Mundo, Derek J. Fisher, Tim I. Miyashiro, Claudia Isabella Pogoreutz, Claudia Isabella Pogoreutz, Claudia Isabella Pogoreutz |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 36 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |