A streamlined and comprehensive protocol for the generation and multi-omic analysis of human monocyte-derived macrophages
Olivia G Palmer, Laurent Perreard, Fred W Kolling IV, Patricia A Pioli, Brittany A Goods · 2025
Read A streamlined and comprehensive protocol for the generation and multi-omic analysis of human monocyte-derived macrophages by Olivia G Palmer, Laurent Perreard, Fred W Kolling IV, Patricia A Pioli, Brittany A Goods (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12752039.txt.
Description
Olivia Palmer and co-authors published in 2025 a full protocol for growing human macrophages from blood monocytes and reading them with modern multi-omic tools. Labs vary wildly in how they wake these immune cells, which muddies comparisons. The team tested four recipes each for the inflammatory and calming states.
Their protocol starts from frozen blood cells, a practical point for biobanks and cell therapy. It details growth conditions, the doses and timing of each waking signal, and checks using gene activity and cytokine output. They also tracked how long the states last after the signals stop. A final trick profiles cell nuclei without prying sticky cells off the dish. The authors aim for macrophage work that travels between labs.
Catalog record
| CREATOR | Olivia G Palmer, Laurent Perreard, Fred W Kolling IV, Patricia A Pioli, Brittany A Goods |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 50 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |