Evaluation and experimental monitoring of calcium in plasma membrane inner nanoscale cytoplasm regions
Sergei M Antonov, Dmitry A Sibarov · 2025
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Description
Antonov and Sibarov measured calcium in a thin layer inside cells for this study from twenty twenty-five. Cells signal with calcium ions, and the zone just under the outer membrane is hard to probe. The team combined a membrane-bound dye with a loose calcium probe in the cell fluid. Two laser settings let them read the two zones apart.
A transfer effect lights the probe only within nanometers of the membrane under one setting. Direct light reads the bulk fluid instead. At rest, the under-membrane zone held strikingly high calcium, above four hundred nanomolar. Signals through channels pushed it higher still. Tests ran in a standard cell line and in neurons. The authors offer the approach as a new tool for calcium nanodomain studies.
Catalog record
| CREATOR | Sergei M Antonov, Dmitry A Sibarov |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 44 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |