Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation
Daseul Yeo, Soontag Jung, Seongwon Hwang, Danbi Yoon, Dong Jae Lim, Songfeng Jin, Jinho Choi, Ki Ho Hong, Changsun Choi · 2025
Read Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation by Daseul Yeo, Soontag Jung, Seongwon Hwang, Danbi Yoon, Dong Jae Lim, Songfeng Jin, Jinho Choi, Ki Ho Hong, Changsun Choi (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12749164.txt.
Description
Tracing hepatitis A outbreaks through food needs the virus full gene code, but food samples hold very little virus and many substances that block the tests. Daseul Yeo and co-authors adapted a standard sequencing workflow for that job in this 2025 study. They worked with clams known to carry the virus, a common outbreak vehicle.
Four tweaks were tried to raise the yield: filtering steps to clean the sample, a special extraction kit, and other preparation changes. The goal was a reliable recipe that outbreak labs can follow on a common sequencing machine. Whole-code reading beats partial tests because it can match cases across towns and pinpoint the source food. The paper reports which tweaks mattered most.
Catalog record
| CREATOR | Daseul Yeo, Soontag Jung, Seongwon Hwang, Danbi Yoon, Dong Jae Lim, Songfeng Jin, Jinho Choi, Ki Ho Hong, Changsun Choi |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 42 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |